apc-cy7-conjugated hamster monoclonal anti-cd48  (Becton Dickinson)

Journal: STAR Protocols

Article Title: Protocol for isolation and analysis of the leukemia stem cells in BCR-ABL-driven chronic myelogenous leukemia mice

doi: 10.1016/j.xpro.2023.102123

Figure Lengend Snippet: Antibody panel for flow cytometry analysis of leukemia stem cells

Article Snippet: APC-Cy7 anti-mouse CD48 antibody , BD Biosciences , Cat#561242; RRID: AB_10644381.

Techniques: Flow Cytometry, Marker

Journal: STAR Protocols

Article Title: Protocol for isolation and analysis of the leukemia stem cells in BCR-ABL-driven chronic myelogenous leukemia mice

doi: 10.1016/j.xpro.2023.102123

Figure Lengend Snippet: Antibody panel for flow cytometry analysis of leukemia progenitor cells

Article Snippet: APC-Cy7 anti-mouse CD48 antibody , BD Biosciences , Cat#561242; RRID: AB_10644381.

Techniques: Flow Cytometry, Marker

Journal: STAR Protocols

Article Title: Protocol for isolation and analysis of the leukemia stem cells in BCR-ABL-driven chronic myelogenous leukemia mice

doi: 10.1016/j.xpro.2023.102123

Figure Lengend Snippet: Gating strategies for analysis of populations of leukemia stem/progenitor cells and myeloid leukemia cells in CML mice by flow cytometry (A) Leukemia stem cells, including GFP + LSK cells (GFP + Lin − Sca-1 + c-Kit + ), GFP + LT-HSCs (GFP + LSK Flt3 − CD150 + CD48 − ), GFP + ST-HSCs (GFP + LSK Flt3 − CD150 − CD48 − ). (B) Leukemia progenitor cells, including GFP + GMP (GFP + Lin − Sca-1 − c-Kit + CD34 + FcγRII/III high ) and GFP + CMP (GFP + Lin − Sca-1 − c-Kit + CD34 + FcγRII/III low ) cells. (C) Myeloid leukemia cells (GFP + Gr1 + ).

Article Snippet: APC-Cy7 anti-mouse CD48 antibody , BD Biosciences , Cat#561242; RRID: AB_10644381.

Techniques: Flow Cytometry

Journal: STAR Protocols

Article Title: Protocol for isolation and analysis of the leukemia stem cells in BCR-ABL-driven chronic myelogenous leukemia mice

doi: 10.1016/j.xpro.2023.102123

Figure Lengend Snippet:

Article Snippet: APC-Cy7 anti-mouse CD48 antibody , BD Biosciences , Cat#561242; RRID: AB_10644381.

Techniques: Recombinant, Cell Culture, Flow Cytometry

Journal: STAR Protocols

Article Title: Protocol to identify and analyze mouse and human quiescent hematopoietic stem cells using flow cytometry combined with confocal imaging

doi: 10.1016/j.xpro.2022.101828

Figure Lengend Snippet: Sample preparation and staining panel—Mouse HSC

Article Snippet: APC-Cy7 Hamster Anti-Mouse CD48; Clone HM48-1 , BD Biosciences , Cat#561242.

Techniques: Sample Prep, Staining, Positive Control

Journal: STAR Protocols

Article Title: Protocol to identify and analyze mouse and human quiescent hematopoietic stem cells using flow cytometry combined with confocal imaging

doi: 10.1016/j.xpro.2022.101828

Figure Lengend Snippet: FACS gating strategy to sort MMP-Low or -High CD48-LSK CD150 + mouse HSCs (A) Determine Lin - CD48 - gate (Gate 1). Flow cytometry APCCy7 contour plots of total BM (Lin/CD48)-APCCy7 positive staining control (i), and Lin - enriched BM full stain sample (ii). (B) Flow cytometry gating scheme towards CD48 - LSKCD150 + from Lin - enriched mouse BM. i, ii, ii, iv, gating debris - (i), doublets - (ii, iii), DAPI - (iv) single viable cells. v, vi, vii, sequential gating of Lin - CD48 - BM (Gate 1; v), Sca-1 + c-Kit + (Gate 2;vi), CD150 + (Gate 3; vii). viii, TMRE Flow histogram of CD48 - LSKCD150 + HSCs. Set bottom 25% MMP-Low and top 25% MMP-High gates.

Article Snippet: APC-Cy7 Hamster Anti-Mouse CD48; Clone HM48-1 , BD Biosciences , Cat#561242.

Techniques: Flow Cytometry, Staining

Journal: STAR Protocols

Article Title: Protocol to identify and analyze mouse and human quiescent hematopoietic stem cells using flow cytometry combined with confocal imaging

doi: 10.1016/j.xpro.2022.101828

Figure Lengend Snippet: Validation of the specificity and responsiveness of TMRE staining to changes in MMP level using CCCP and oligomycin Flow cytometry histogram of TMRE for CD48 - LSKCD150 + HSCs treated with CCCP (100 μM, red), or oligomycin (2 μg/mL, blue), or untreated (DMSO, 10 min, black). Depolarized and hyperpolarized TMRE gates were determined according to the peak separation on TMRE profile of CCCP and oligomycin treated samples respectively. Only 0.96% or 1.44% untreated HSCs are depolarized or hyperpolarized. TMRE intensity of 97.6% of cells are within physiological range.

Article Snippet: APC-Cy7 Hamster Anti-Mouse CD48; Clone HM48-1 , BD Biosciences , Cat#561242.

Techniques: Staining, Flow Cytometry

Journal: STAR Protocols

Article Title: Protocol to identify and analyze mouse and human quiescent hematopoietic stem cells using flow cytometry combined with confocal imaging

doi: 10.1016/j.xpro.2022.101828

Figure Lengend Snippet:

Article Snippet: APC-Cy7 Hamster Anti-Mouse CD48; Clone HM48-1 , BD Biosciences , Cat#561242.

Techniques: Recombinant, Cell Isolation, Selection, Software